Paralytic Shellfish Poison (PSP) are the most harmful neurotoxins create a serious public health problem. It is important to assess PSP in Shellfish destined for human consumption. However, recommended methods have some limitations for example in the case of Mouse Bioassay (MBA) showed a low sensitivity and reproducibility, and undesirability for ethical reasons; while physico-chemical techniques rest expensive and time-consuming. The main objective of this study, after discovered that PSP inhibited the luminescence of Vibrio fischeri, was the quantification of PSP by using Bioluminescence Inhibition Assay (BIA), and comparing the results obtained with those determined by MBA and LC-MS. Bivalve used were collected from Corniche Martil, Kabila and Oued Laou, along the Mediterranean coast of Morocco in Mars-2015. Results showed a weak correlation between LC-MS and MBA with r = 0.11, while, the correlation between LC-MS and BIA was very strong with r = 0.97, which suggests that, BIA could offer an interesting additional assessment of PSP risk. In addition, after seen its rapidity, ease, reliability, sensitivity, reproducibility and cost effectiveness, it would be eligible to use for monitoring in surveillance programs.
Key words: Method, Bioluminescence, Vibrio fischeri, Acanthocardia Tuberculatum, bivalve, PSP, STX
|
