Home|Journals|Articles by Year|Audio Abstracts
 

Original Research



Antitrypanosomal, antiplasmodial, and antibacterial activities of extracts from selected Diospyros and Annonaceae species

Robert Christopher, Quintino A. Mgani, Stephen S. Nyandoro, Amanda L. Rousseau, Sandy F. van Vuuren, Michelle Isaacs, Heinrich C. Hoppe.




Abstract

Aim: To screen methanol extracts from root bark, leaves, and stem bark of selected plant species from the genus Diospyros and some Annonaceae species for antitrypanosomal, antiplasmodial, and antibacterial activities against selected test organisms.
Methods: Antitrypanosomal and antiplasmodial assays of methanol extracts from selected plant species were carried out in single concentration screens and in dose-response for active extracts. The minimum inhibitory concentration (MIC) values of selected plant extracts against selected bacterial strains were determined by microplate dilution method in sterile 96-well microtiter plates.
Results: In the dose-response antitrypanosomal assay, the most potent extracts tested exhibited activities against Trypanosoma brucei brucei (Lister 427 strain) with IC50 values ranging from 1.28 to 7.85 μg/ml, with methanol extract of Diospyros verrucosa stem bark being the most active with IC50 value of 1.28 μg/ml. In the dose-response antiplasmodial assay, three extracts exhibited activities against Plasmodium falciparum(strain 3D7) with IC50 values ranging from 4.55 to 24.22 μg/ml, with methanol extract of Diospyros capricornuta root bark being the most potent with IC50 value of 4.55 μg/ml. In the antibacterial assay, the investigated extracts exhibited a wide range of activities against Staphylococcus aureus [American Type Culture Collection (ATCC) strain 25923], Bacillus cereus (ATCC strain 11775), and Escherichia coli (ATCC strain 8740) with MIC values ranging from 0.00125 to 0.00625 mg/ml (more active), 0.125 to 0.500 mg/ml (moderately active), and 1.00 to 8.00 mg/ml (less active) while some extracts were inactive at the highest concentration tested of 16.00 mg/ml.
Conclusions: Methanol extracts obtained from root bark, leaves, and stem bark of selected plant species from the genus Diospyros and some Annonaceae species that showed good activities in antitrypanosomal, antiplasmodial, and antibacterial assays corroborate reported literature about the traditional medicinal uses of the members of genus Diospyros and some Annonaceae species.

Key words: Antitrypanosomal screening, antiplasmodial screening, antibacterial screening.






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.