ABSTRACT
Susceptibility of some gastrointestinal tract (GIT) bacterial-pathogens to combinations of MRSA-growth inhibiting antibiotics (MGIA), MRSA growth non-inhibiting antibiotics (MGNIA) and some plant extracts, separately or in groups were investigated. The results revealed that combinations from MGNIA (Gentamycin with Co trimoxazole), MGIA (Vancomycin with Ciprofloxacin) and MGNIA with MGIA (Gentamycin with Imipenem) were determinative for the growth of most tested GIT-pathogens. On the other hand, aerial shoots of Artimesia monosperma L (Am), Ocimum basilicum L (Ob), Origanum majorana L (Om), Salvia officinalis L (So) and Pelargonium graveolens (Pg) were applied collectively in a mixture (PM) and yield the most lethal effect on the tested pathogens (13.2 IZD). Further total plant mixture (TPM) Foeniculum vulgare (Fv) seed mixture (SM) with PM increased the determinative impact on pathogens growth and resulted in more susceptibility (17.3mm IZD). GC/MC analyses have shown that the major essential oils compounds of TM were β -Pinene 55 %, from (Am), Linalool 46%, Methyl chavicol 14.2% and 1, 8- cineole 12.3% from (Ob), terpinen-4-ol 31% and γ-Terpinene 14% from (Om), camphor 26.4 %, 1, 8- cineole 18% and α-thujone 14% from (So). Citronellol 40% from (Pg), Trans-anethole 66% and 1, 8- cineole12% from (Fv). Moreover, combination of TPM with Gentamicin and Imipenem (TPMGI) had a further upmost synergistic effect (25 mm IZD). The mode of action for TPMGI mixture ingredients may be resulted from the disruption of bacterial cell membrane, blocking of protein synthesis and out diffusion of cellular components.
Key words: MRSA, antibiotics, GIT-pathogens, Essential oils, medicinal plants.
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