Abstract

The aim of this study was is to isolate and characterize new Egyptian marine bacterial strains and to evaluate the antimicrobial, antioxidant and total phenolic content of their bioactive secondary metabolites. Eight bacterial strains were isolated from marine sediment samples collected from different areas in Red sea governorate, Egypt. Antifungal activity was recorded as zone inhibition of growth between the fungus and the organism tested and two bacterial isolates were selected (designated 21 and 30). These isolates significantly reduced fungal growth and AFB1 production especially the isolate 30. These promising isolates were selected and identified using molecular identification technique and identified as Brevundimonas and Bacillus. The crude extracts from the two bacteria were extracted and the biological activity of these crude extracts was studied. The crude extracts exhibited potent antimicrobial activities against a set of microbial pathogens as well as antioxidant activity and total phenolic content.

Key words: alkaliphilic bacteria, AFB1, PCR, 16S rRNA, antimicrobial activity, antiaflatoxigenic activity, antioxidant capacity, total phenolic content,