Aim: Acrosin is a unique trypsin-like acrosomal proteinase that is required for mammalian fertilization.
Nitrosamines are a large group of chemical compounds that have been found to be carcinogenic in all species of
animals tested and thus suspected to be a human carcinogen. The purpose of the our study was to investigate the
in vitro effect of nitrosamine, on human acrosin activity and some of the main amino acids (tryptophan,
phenylalanine, histidine, tyrosine, pyroline, cysteine, methionine and arginine),
Material and Methods: In vitro effect of nitrosamine, on human acrosin activity and some of the main amino acids,
which are responsible for the constitution of acrosin, was investigated by thin layer chromatography.
Results: Our results showed that nitrosamines can have non-competitive inhibitory role on enzyme activity.
Results of effect of nitrosamines on some of the main amino acids showed that 24 hours incubated amino acidnitrosamine
mixtures areas were smaller then free amino acid, free NMOR and free NPYr, indicating that
reaction velocity was increased as in a time-dependent manner, therefore the amount of product was increased,
Conclusion:It was observed that the reaction velocity increased with time and that the resulting product increased
with time. In other words, binding of amino acids to nitrosamine increases in a time depended manner. The
present results indicated the non-competitive inhibitory effect of nitrosamines on acrosin.
Key Words: Human acrosin, Thin layer chromatography, Nitrosopyrrolydine, Nitrosomorpholine, Enzyme kinetic, Amino acid.
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