Home|Journals|Articles by Year|Audio Abstracts
 

Original Research

Egypt. J. Exp. Biol. (Bot.). 2012; 8(1): 107-111


THE CORRELATION BETWEEN BIOFILM FORMATION AND RESISTANCE TO ANTIBIOTICS IN STAPHYLOCOCCAL INFECTIONS

Hala M. AbuShady Rasha A. Nasr Zeinab E. Hasan Hussein S. Hussein.




Abstract

Thirty one staphylococci out of 50 were BF forming (according to icaAD gene detection by polymerase chain reaction and/or microtitre plate assay) and 19 non-BF forming isolated from clinical specimens and intravascular catheters of patients admitted to the pediatric hospital of Ain-Shams University. Antibiotic susceptibility testing was performed for all isolates using disk diffusion test for cefoxitin, gentamicin and rifampicin and HiComb MIC Test for vancomycin, erythromycin, ciprofloxacin, sulphamethizole and teracycline. An increase in the resistance of the staphylococcal isolates, particularly the coagulase negative, was noted. No statistical significant difference was detected between BF forming and non BF Staphylococci, however, 48.8% of BF forming strains were methicillin resistant (MR) and all were associated with multiple-resistance to antibiotics as 16% were resistant to 3 antibiotics, 32% to 4 antibiotics, 39% to 5 antibiotics, and 13% to all antibiotics tested. A high resistance was detected MR staphylococcal isolates to erythromycin (92.9%), gentamicin (60.7%), and ciprofloxacin (89.3%) showing a statistical significant difference when compared to methicillin sensitive Staphylococci (63.6%, 9%, 66.7%, respectively). Finally we conclude that there is an increase in the resistance of staphylococcal isolates to different antibiotics and BF formation is associated with multi-resistance to antibiotics.

Key words: Staphylococci, Biofilm, Antibiotics, Disk Diffusion, Minimal Inhibitory Concentration






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.