Objectives of current study were to identify single nucleotide polymorphisms (SNP) in heat shock protein70 (Hsp70) promoter gene of bulls (cattle and buffalo) and its association with semen quality traits and some enzymatic, hormonal and lipid parameters during winter and summer seasons. Semen samples of 37 animals (Cattle n=21 and buffalo n=16), were used in the study. Biochemical Parameters and hormonal profile were estimated. Qualities of fresh semen and frozen-thaw semen were evaluated for motility, plasma membrane and acrosome integrities. PCR-SSCP was used to detect SNPs and different patterns were sequenced. The study demonstrate all the measured biochemical parameters and hormonal profile of cattle and buffalo were significantly different (PC transition was the same in both species and could clear two genotypes TT and TC. Association analysis revealed significance (P
Key words: HSP70, Polymorphism, Biochemical parameter, Hormonal profile, Semen quality
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