Pseudomonas is a common cause of nosocomial infection and may lead to neonatal septicaemia. The present in vitro study was planned to understand the rationality of using the Suwarnaprash, Suwarnaprash blended with fresh fruit's extract or powder of Amalaki (Emblica officinalis Gaertn.) in addition to components of various Prash. The Suwarnaprash is an amalgamation of incinerated Swarna bhasma, honey and clarified butter oil (CBO).
The life-threatening pathobiont, i.e. Pseudomonas aeruginosa was cultured on the sterile MuellerHinton agar plate surface and kept in an incubator overnight (24 hours). The Aamlaki powder, fresh extract of Aamlaki fruits, distilled water-diluted Swarna bhasma, pure honey, clarified butter oil, Suwarnaprash, Suwarna-Aamlaki prash-I consisting powder of Aamlaki with Suwarnaprash and Suwarna-Aamlaki prash-II includes fresh extract of Aamlaki with Suwarnaprash were inoculated on lawned petri plates in a quantity of 10 µl, 10 µl,10 µl, 0.04 g (~1 drop), 10 µl, 0.2 g, 0.2 g and 20 µl, respectively.
The amalgamation of powder of Emblica officinalis with Suwarnaprash and the Amalaki powder (Emblica officinalis) have shown 20 mm inhibitory zones compared to the 10 mm inhibitory zone of Suwarnaprash.
The result suggests that Suwarna-Amalaki prash-I and Amalaki (Emblica officinalis) powder are more potent to inhibit the growth of Pseudomonas aeruginosa in vitro than any other trial sample. Therefore, Suwarna-Amalaki prash-I may be considered a Suwarnaprash of choice for preventing Pseudomonas aeruginosa induced septicaemia than the Suwarnaprash alone under the Jatakarma Samskara as an additional property.
Key words: Emblica officinalis, Suwarna-prash, Antipseudomonal activity, Disk diffusion method
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