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Original Article

J. Islam. Int. Med. Coll.. 2018; 13(2): 85-90


Comparative Evaluation of CHROMagar and API 20C AUX in Isolation and Identification of Candida Species

Uzma Mussarat Malik, Abdul Bari Khan, Muhammad Luqman Satti.


Abstract

Objective: To evaluate the performance of CHROMagar and API 20C AUX for the documentation of different
Candida species.
Study Design: Descriptive cross-sectional study.
Place and Duration of Study: The study was conducted in the Department of Microbiology at AFIP (Armed
Forces Institute of Pathology), CMH Rawalpindi and Army Medical College Rawalpindi in collaboration with
Departments of Pathology (Microbiology) at Pakistan Railways Teaching Hospital (PRH), Islamic International
Medical College Rawalpindi from 01th April 2017 to 30th September 2017.
Materials and Methods: Collectively 100 isolates of candida yielded from HVS clinical samples. Phenotypic
tests including growth on CHROMagar Candida and API 20C AUX were used for reporting different Candida
species. Clinical Candida isolates along with reference institutional control strains of Candida species were used
in the study. Data was analyzed using simple descriptive statistics (frequencies, percentages) for each
categorical variable.
Results: Among 100 candida isolates 92 (92%) isolates of Candida were identified correctly to level of species by
CHROMagar Candida, in comparison to 100% identification of candida species using API 20C AUX. Results of
present study revealed that CHROMagar Candida can be used to report three species of Candida considering
the morphology and colour of colonies of these particular species, and to distinguish them as C. albicans, C.
tropicalis, and C. glabrata.
Conclusion: Both phenotypic tests CHROMagar plates and API 20 C AUX are effective in the documentation of
Candida species. However API 20 C Aux is found to be more accurate than CHROMagar because less commonly
isolated Candida species cannot be documented using CHROMagar. Moreover being less costly; use of
CHROMagar Candida is helpful in rapid identification and constructing suitable therapeutic plan for patient's
management in laboratories with limited resources.

Key words: Candida Species, HVS, Identification Methods.






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