Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Hepatoprotective effect of melatonin via activation of Nrf2 and anti-apoptotic proteins in burn rats

Ganka Bekyarova, Minka Hristova, Maria Tzaneva, Andrey Kotzev.


Warning: mysql_num_rows() expects parameter 1 to be resource, boolean given in /home2/bibliomed/depo/functions_site.php on line 126

Abstract
Abstract
Objective: Burn- induced acute hepatic injury due to increased production of lipid peroxides and increased cellular apoptosis. The nuclear factor erythroid 2-related factor 2 (Nrf2) pathway is essential for cytoprotection against oxidative stress. We hypothesized that melatonin by activation of Nrf2 may shift Bax/Bcl-2 ratio to protect rat hepatocytes against apoptosis and progressive liver injury. The aim of this experimental study was to investigate the protective effects of melatonin against burn-induced apoptotic injury and the relationship between lipid peroxides expression of transcription factor Nrf2 and apoptotic protein in burn rat model. Methods: Melatonin was applied immediately after the burn. The expression of hepatic 4-hydroxynonenal (4-HNE), as a marker of liver peroxidative injury, Nrf2, as a marker of antioxidant defense and apoptosis-related genes Bcl-2 and Bax were evaluated using light immunŠ¾histochemistry. Results: Burns caused an increased expression of 4-HNE, Bax and Bax/Bcl-2 ratio and induced apoptosis of sinusoidal endothelial cells (SECs) in liver tissue. Melatonin treatment augmented the increase in Nrf2 expression, decreased both burn-induced peroxidative damage and hepatic apoptosis as evidenced by reduced expression of Bax, enhanced expression of Bcl-2. In conclusion, our data suggest that activation of transcription factor Nrf2 by melatonin is protective against oxidative stress, apoptosis and hepatic injury in burns. The available information by melatonin`s effect on the redox sensing transcription factor Nrf2, as a regulator of antioxidant enzymes, antioxidants and antioxidant protection of the liver is limited. Melatonin activates the Nrf2/signaling pathway and acts as a natural inducer of anti-apoptotic and antioxidant protection under condition of burn-induced oxidative stress. This is a new cellular mechanism for protection against progressive burn-induced liver damage not only in animals but also in humans.

Key words: Key words: melatonin, liver, Nrf2, Bax, Bcl-2, burns



Full-text options

Full-text Article



Readers of this article also read the following articles
»The effect of maternal anthropometric characteristics and social factors on birth weight of child in small town hospital of Gandevi block of Navsari district.
»Shifting trends of HIV epidemiology among most at risk groups (MARGs) in India
»Toxicities due to highly active anti-retroviral therapy (HAART) in HIV-positive children: need for further medical research
»Prevalence of overweight and obesity in affluent adolescent girls in Surat city, western India
»Sample size in clinical research, the number we need
»Delayed onset renal failure following multiple bee stings
»Beware – statistics may deceive you
»Why one more journal?
»A study of sacrum with three pairs of sacral foramina in western India
»Need of medicines information OPD in tertiary health care settings: A cross sectional study
»Prevalence of mosquito-borne Plasmodium infection in patients attending fever clinic in a tertiary-care teaching hospital, western India



Advertisement
Applied Medical Research

SUBMIT YOUR ARTICLE NOW




ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.