Home|Journals|Articles by Year Follow on Twitter| Subscribe to List

Directory for Medical Articles

Open Access

Original Article

Hepatoprotective effect of melatonin via activation of Nrf2 and anti-apoptotic proteins in burn rats

Ganka Bekyarova, Minka Hristova, Maria Tzaneva, Andrey Kotzev.

Objective: Burn- induced acute hepatic injury due to increased production of lipid peroxides and increased cellular apoptosis. The nuclear factor erythroid 2-related factor 2 (Nrf2) pathway is essential for cytoprotection against oxidative stress. We hypothesized that melatonin by activation of Nrf2 may shift Bax/Bcl-2 ratio to protect rat hepatocytes against apoptosis and progressive liver injury. The aim of this experimental study was to investigate the protective effects of melatonin against burn-induced apoptotic injury and the relationship between lipid peroxides expression of transcription factor Nrf2 and apoptotic protein in burn rat model. Methods: Melatonin was applied immediately after the burn. The expression of hepatic 4-hydroxynonenal (4-HNE), as a marker of liver peroxidative injury, Nrf2, as a marker of antioxidant defense and apoptosis-related genes Bcl-2 and Bax were evaluated using light immunŠ¾histochemistry. Results: Burns caused an increased expression of 4-HNE, Bax and Bax/Bcl-2 ratio and induced apoptosis of sinusoidal endothelial cells (SECs) in liver tissue. Melatonin treatment augmented the increase in Nrf2 expression, decreased both burn-induced peroxidative damage and hepatic apoptosis as evidenced by reduced expression of Bax, enhanced expression of Bcl-2. In conclusion, our data suggest that activation of transcription factor Nrf2 by melatonin is protective against oxidative stress, apoptosis and hepatic injury in burns. The available information by melatonin`s effect on the redox sensing transcription factor Nrf2, as a regulator of antioxidant enzymes, antioxidants and antioxidant protection of the liver is limited. Melatonin activates the Nrf2/signaling pathway and acts as a natural inducer of anti-apoptotic and antioxidant protection under condition of burn-induced oxidative stress. This is a new cellular mechanism for protection against progressive burn-induced liver damage not only in animals but also in humans.

Key words: Key words: melatonin, liver, Nrf2, Bax, Bcl-2, burns

Full-text options

Full-text Article

Share this Article

Readers of this article also read the following articles
»Impact of exposure to biomass on the vascular function of Senegalese women
»Supra-Clavicular Brachial Plexus Block: Ultra-Sonography Guided Technique Offer Advantage Over Peripheral Nerve Stimulator Guided Technique
»Aortic surgery cost and outcome at teaching vs. non-teaching hospitals
»Regulation of Physical Therapy Profession in Pakistan
»Hand-Washing Practices of Women; a Qualitative Study
»The Effects of Mood Disorders and Dissociative Symptoms on Suicide Attempts
»Dirty Dozen Scale: a study of adaptation to Turkish university students
»Enrichment of protein content in cassava (Manihot esculenta Crantz) by
supplementing with yeast for use as animal feed
»Validity and reliability for the Turkish adaptation of the Consciousness in Dreams Scale
»Design and Development of a Clinical Risk Management Tool Using Radio Frequency Identification (RFID)

American Journal of Physiology, Biochemistry and Pharmacology


BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.