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Review Article

SRP. 2020; 11(6): 858-863

Inhibitory Power Test of White Rice Bran Extract (Oryza sativa L.) with the Solution of Ethanol and Aquades on Porphyromonas gingivalis (In Vitro) Bacteria

Harun Achmad, Marhamah F. Singgih, Huldani, Al Frida Ramdhani, Yunita Feby Ramadhany.

Background: Bran is byproduct of the rice milling process and rice
waste. Out of 32 million tons of rice obtained by rice bran as a
byproduct of around 2,5 million tons. White rice bran (Oryza sativa L.)
has many bioactive ingredients, one of which is a phenol compound.
Phenol compound are known to have antibacterial activity.
Porphyromonas gingivalis (p.gingivalis) is one of Gram negative
anaerobic that contribute to the occurrence of periodontal disease, so
that materials that have antibacterial properties are very necessary to
be given to inhibit the growth of these bacteria.
Objectives: Knowing the inhibitory effect of white rice bran extract
(Oryza sativa L.) with ethanol and aquadest solvents to P.gingivalis
bacteria at concentrations of 12,5%, 25%, 50%, 75% and to
determine the difference in the effectiveness of white rice bran
extract (Oryza sativa L.) with ethanol and aquadest solvents to
P.gingivalis bacteria.
Methods: This research was laboratory experiment with post test only
control group design with diffusion method Kirby Bauer. In this
research with 6 repetitions with extract at concentrations of 12,5%,
25%, 50%, 75%, positive control (Chlorhexidine), and negative control
(aquadest). The measuring instrument in this research was using
caliper with millimeters (mm). Analysis data was using Krusskal Wallis
continued by Mann Whitney test.
Results: The results showed that white rice bran extract at
concentrations of 12,5%, 25%, 50%, 75% with ethanol and aquadest
solvents are significantly influence (Krusskal Wallis test showed p
value = 0,000 (p

Key words: White rice bran extract (Oryza sativa L), Antibacterial, Ethanol, Aquadest, Porphyromonas gingivalis

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