|SRP. 2020; 11(11): 281-284
Virus-Reproducing Ability of Growth Media Containing Immunogenesis Mediators
Edie M. Plotnikova, Irina A. Nesterova, Ramzi N. Nizamov, Zoya G. Churina, Andrey I. Samsonov, Elena L. Matveeva, Gennady N. Spiridonov, Aidar F. Makhmutov, Konstantin N. Vagin, Ruslan R. Gainullin.
Background and Aim: The article is dedicated to the study of the possibility of use of cytokine stimulated continuous cell lines MDBK and BHK-21/13-02 for reproduction of PI-3 (parainfluenza-3) and IRT (infectious rhinotracheitis) viruses in order to increase viral biomass for production of antiviral vaccines and diagnostic test systems.
Materials and Methods: Cell cultures MDBK and BHK-21/13-02, Eagle MEM growth medium, cattle blood serum were used as biological model in experiments, and IRT and PI-3 viruses were used as test viruses, and interleukin 6 was applied as a potential cell culture metabolism activator.
Results: The study established that from 8 variants of experiments using various combinations of growth media, cell cultures and viruses, cell cultures MDBK and BHK-21/13-02 incubated in the medium with addition of combination of cattle serum and interleukin-6 at a concentration of 60 pg/ml are optimal for the reproduction of the IRT and PI-3 viruses. In addition, the viral activity was 1.24-1.26 lg TCD50/ml higher than in the presence of cattle serum only in growth medium.
Conclusion: Therefore, it was established that the use of cytokine-stimulated cell cultures MDBK and BHK-21/13-02 contributes to increased reproduction of IRT и PI-3 viruses and makes possible to obtain a considerable viral mass with sufficiently high infectious activity.
Key words: cytokines, cell cultures, IL-6 (interleukin), viruses, reproduction