Objective: To compare the hepatoprotective effects of aqueous extract of Cassia fistula leaves and Silymarin on
Acetaminophen induced hepatotoxicity.
Study Design: An experimental study in mice.
Place and Duration of Study: The study was conducted in the department of Pharmacology and Therapeutics,
Multidisciplinary research laboratory of Islamic International Medical College, Riphah International University.
The duration of study was from 16 Mar, 2016 to 16 Mar, 2017.
Materials and Methods: In present study simple random sampling was done by Balloting method and sixty
adult Balb/c Albino mice were divided in four groups having 15 mice each. Mice in control group A were given
normal diet and fresh water. Mice in experimental group B (positive control) were given Acetaminophenat dose
of 100mg/kg/day orally for six weeks. Mice in experimental group C were treated with
Acetaminophen(100mg/Kg) with concurrent 400mg/Kg body weight of Cassia fistula (Aqueous extract) orally
for six weeks once daily while in experimental group D mice received 750 mg/kg body weight of Silymarin orally
in form of aqueous suspension in 2% Cremophor1E for 6 weeks once daily concurrently treated with
Acetaminophen(100mg/Kg).The cardiac puncture was done after six weeks and 2.5cc blood was collected
from each mice for assessment of biochemical markers including Serum Alanine Aminotransferase (ALT) and
Serum Aspartate Aminotransferase (AST).
Results: The mean value of serum ALT level was 130.4.30 ±42.90 in Group B as compared to control Group
having mean value of ALT 37.30 ± 8.32. The mean value of ALT was 62.22± 19.27 and 53.90± 25.06 in Group C
and in Group D respectively. In Group B mean value of serum level of AST was 134.3± 15.56 as compared to
control Group A having mean value of AST31.40± 10.05.The mean value of AST in Group C and Group D were
66.40±20.50 and 44.67± 20.37 respectively.
Conclusion: The Silymarin is more beneficial than aqueous extract of Cassia fistula leaves in ameliorating
hepatotoxic effects of Acetaminophen.
Key words: Acetaminophen, Cassia fistula leaves, Hepatoprotective, Silymarin.