Home|Journals Follow on Twitter| Subscribe to List

Directory for Medical Articles
 

Open Access

Original Article



Comparison of two different applications of the Griess method for nitric oxide measurement

Aysegul A. Yucel, Sebnem Gulen, Sibel Dincer, Ahmet E. Yucel, Gulay I. Yetkin.

Cited by (5)

Abstract
Objective: Nitric oxide (NO) is produced by various cells in response to mitogenic and inflammatory stimuli. This molecule plays important roles in the transmission of cellular signals, and in defense against pathogens by oxidative toxicity. In biological fluids, NO is rapidly converted to nitrite and nitrate. Therefore, the best index of total NO production is the sum of nitrite and nitrate. The most widely accepted classical method for measuring NO is the Griess method. Widely used commercial colorimetric kits involving the Griess method are very costly. In this study, we aimed to compare two different applications of Griess method for NO measurement.
Methods: We tested two identical sets of 155 human plasma samples for NO with two different applications of the Griess method. These applications involved different deproteinization and nitrate-nitrite conversion methods. The fırst application was a commercial (Cayman Chemical) NO colorimetric assay with deproteinization via ultrafiltration and nitrate-nitrite conversion using NADPH-dependent nitrate reductase, and the second one was an in-house application involving sulphanilamide and N-(1-naphthyl) ethylendiamine dihydrochloride compounds with NaOH and ZnSO4 deproteinization and VaCl3 was used for nitrate-nitrite conversion.
Results: The results with the se two different Griess applications were statistically correlated (r=0.954, p

Key words: Griess; NaOH; Nitrate reductase; Nitric oxide; Ultrafıltration; VaCl3; ZnSO4



Share this Article


Advertisement
Journal of Behavioral Health

SUBMIT YOUR ARTICLE NOW


ScopeMed.com
BiblioMed Home
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright © ScopeMed® Information Services.