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Original Article



Investigation of Patients with Suspected Cystic Echinococcosis by ELISA and IHA Methods in Gaziantep, Turkey

Ahmet Ozkeklikci, Eylem Akdur Ozturk, Mesut Akil, Aysegul Unver, Nuray Altintas.




Abstract

Objective: Cystic echinococcosis (CE) caused by larval stage of Echinococcus granulosus is one of the neglected parasitic disease, of both medical and public health importance. It has a worldwide distribution including Turkey. The current study was designed to determine the seropositivity of E. granulosus infection in patients with suspected cystic echinococcosis attending to Dr.Ersin Arslan Training and Research Hospital in Gaziantep, Turkey.
Materials and Methods: A total of 545 serum samples were taken from individuals were screened for the presence of E. granulosus IgG antibodies using in-house Enzyme-Linked Immunosorbent Assay (ELISA) and the commercial Indirect Hemagglutination Assay kit (IHA-Fumouze kit). Furthermore, socio-demographic characteristics as gender, age, residence were analyzed by SPSS.
Results: Out of 545 samples, antibodies to E. granulosus were detected in 148 (27.2%) patients with ELISA and 83 patients (15. 2%) were found positive with IHA test. Statistical comparison of two dependent categorical values (ELISA and IHA) showed statistical significance p

Key words: Cystic echinococcosis, Echinococcus granulosus; serodiagnosis; hydatid fluid; Enzyme Linked Immunosorbent Assay; Indirect Hemagglutination Assay






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