Buffaloes productivity is affected mainly due to many inherent disorders. Estrogen hormone is found to be a important hormone directly linked with reproductive functions of buffalo. The present study was performed with 203 Murrah / Graded Murrah buffaloes maintained at different locations. The Polymerase Chain Reaction- Single Strand Conformation Polymorphism (PCR-SSCP) of ER-α gene revealed monomorphic pattern for all the tested animals, which indicates the fixation of allele in tested buffalo population. As a conclusion, monomorphic pattern of Estrogen Receptor-α (ERα) gene is considered a unique feature that may be related to the characteristic of buffaloes.
Key words: Buffalo, PCR-SSCP, ERα gene, Monomorphism