Linum usitatissimum (L.), the flax and Sesamum indicum (L.), the sesame seeds are rich sources of lignans, SDG and Sesamin (Sm) respectively. Synergistic effects of the SDG and Sesamin (Sm) lignan samples were investigated in the present study. The HPLC fingerprint identified the presence of secoisolariciresinol diglucoside (SDG) and sesamin in seeds of flax (FS) and sesame (SS). Antimicrobial activity of SDG + Sm combination by disc diffusion against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus subtilis significantly arrests bacterial growth in comparison to independent use of SDG and Sesamin. Bacterial biofilm inhibition capacity of SDG+Sm imaged by Confocal laser scanning microscopy revealed the loss of microcolonies. SDG+Sm could inhibit the 15-LOX and COX-2 enzyme at relatively lower concentrations. Further, SDG+Sm quenched free radicals produced by Fentons reagent studied by DNA-nicking assay indicating its strong antioxidant property with the capacity to protect DNA. These results put together, encourage the use of a combination of flax and sesame seed lignans SDG+Sm in a wide range of applications as natural preservatives with pharmacological effects such as anti-inflammatory agent and aid in their promotion as nutraceuticals.
Key words: SDG; Sesamin; collegial effect; CLSM; biofilm;