The Boraginaceae family comprises around 2000 species within 145 genera with various therapeutic and pharmacological attributes. DNA banking can be considered a complementary method for preserving plant species by preserving genomic DNA at lower temperatures. This approach to maintaining biological information allows researchers to search for new genes and their products. Therefore, in this review, we describe and analyze the DNA barcoding of family Boraginaceae to conserve and further manipulate this rare and endangered plants species to enhance its yield and quality characteristics. The non-coding DNA regions (such as ITS and rbcL) are presumed to be more useful at lower taxonomic levels because of their faster evolutionary rates. The present study aims to inspect whether these plants can be categorized into different species or not, and DNA barcoding is used to clarify the dispute. The DNA barcode marker used ITS and rbcL, and sequence analysis was performed using the online tool BLAST of the NCBI database and based on maximum identity and dendrogram.
Key words: Boraginaceae, DNA barcoding, plastid genome, phylogenetic analysis, molecular marker Elba mountain, South Egypt.
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