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Molecular identification of Halophilic Microorganisms producing Exo-Halozymes by a culture-dependent approach from the North Sinai Solar saltern

Naglaa F Elshafey, Aya A Elkashef, Hend A Hamedo, Mohamed A. I. Mansour.




Abstract

Solar salterns are an ideal model for extreme environments since they contain a halophilic microorganisms that must adapt to these conditions. In North Sinai, these environments have not gotten much attention. However, the focus of our investigation was on the salterns' waters and saline soil. The study of this microflora showed that it had a lot of different shapes, functions, and ways of using energy. The molecular analysis provided us with a phylogenetic relationship that included Aspergillus isolates as well as archaeal isolates from the genera Haloarcula, Haloferex, and Halobacterium, and also bacteria related to Halomonas and Brevibacillus. The majority of isolates expressed hydrolytic enzymes at high salt concentrations. Finally, the North Sinai Solar Saltern is rich in halophilic populations that produce industrial hydrolytic enzymes.

Key words: Halophilic community, Halozymes, solar saltern, North Sinai






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