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Review Article

SRP. 2020; 11(2): 273-277


Production Technique of Bifido bacterium’s Exo-metabolites with High Antimicrobial Activity towards Staphylococcus aureus

Markov A.A, Timokhina T.H, Perunova N.B, Malyugina O.A.




Abstract

Results of conducted development of the way for obtaining Bifidobacterium bifidum’s (B. bifidum) exo-metabolites with high antimicrobial activity are shown in this article. The suggested method of obtaining of B. bifidum exo-metabolites with high antimicrobial effect includes the preparation of nutritious environment, refrigeration to the temperature of cultivation, deep introduction of bifido bacterium’s cultures, and cultivation with further excretion of B. bifidum metabolites. Reference culture of Staphylococcus aureus АТСС 25923 was grown on Muller Hinton agar during 24 hours under 370 С. The source suspension of Staphylococcus aureus АТСС 25923 was prepared in sterile isotonic solution (0,5 MF - 1,5х108 cfu/ml). During the determination of bactericidal activity of Bifidobacterium bifidum daily culture’s exo-metabolites towards test strain of Staphylococcus aureus ATCC 25923, it was proved that two-day exo-metabolites of Bifido bacterium bifidum showed full absence of growth of against to Staphylococcus aureus 25923 ATCC by the way of the most bactericidal activity. This method provides the obtaining of Bifidobacterium bifidum’s exometabolites with high antimicrobial activity and the research of possibilities of wide appliance in medicine.

Key words: Bifidobacterium bifidum, exometabolites, antimicrobial activity, Staphylococcus aureus.






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