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RMJ. 2005; 30(1): 5-8

Measurement of Optical Parameters: Absorption Scattering and Auto-florescence of Skin in Vitro

S.Firdous, M.Ikram, M.Nawaz* and M.Aslam*..

Objective: To measure various optical parameters in skin sample of chicken.
Methods: We illuminated the chicken breast skin tissues with collimated radiation of 400-700 nm Nd-YAG pumped dye laser, and measured skin optical properties for dry and hydrated sample in vitro. Total reflectance and transmitted intensities were recorded by which scattering, absorption and anisotropic factors of the sample obtained using double integrating sphere setup.
Results: The in vitro optical parameters are higher then in vivo measurements. Hydration of skin is found to influence its scattering properties. Dry sample scatter less then hydrated sample.
Conclusion: Our in vitro results are in agreements with other data available in literature.
(Rawal Med J 2005;30:5-8).

Key words: Optical imaging, light transmittance, autofluorescence.

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