The article studies the influence of natural and extreme conditions of the aquatic environment on the development and symbiosis of a strain of the unicellular planktonic green microalgae Chlorella vulgaris BIN, 6-10 microns in size. The studies were carried out in 2019, 2020 on the basis of laboratory analysis of samples taken at a depth of 10 cm from 15 control points of the Nizhny Fermsky pond of the Federal State Budgetary Educational Institution of Higher Education RSAU-Moscow Agricultural Academy named after K.A. Timiryazev, and in an open aquarium (bioreactor) with a volume of 50 liters in which, using the Arduino hardware and software complex, the temperature and lighting optimal for the growth of microalgae of the Chlorella vulgaris BIN strain were automatically maintained, as well as biochemical and physical conditions for the growth and development of microalgae were simulated strain Chlorella vulgaris BIN. In a separate study, completed in January 2020, the development and symbiosis of chlorella with unicellular and multicellular aquatic organisms was studied in an open aquarium with artificial lighting and temperature maintenance, in the presence of a critically large amount of macro and microelements used by the microalga of the Chlorella vulgaris BIN strain at the growth and reproduction. To calculate the number of chlorella cells in the samples, a Goryaev two-grid camera was used, in which the identification and quantitative accounting of chlorella cells was carried out according to the results of photographs and video filming, with the resolution settings of the Bresser LCD MICRO 5MP digital microscope at 125, 500 times, and at 1000 times electronic ZOOM magnification. As a result of the work carried out, a number of limiting factors have been identified that affect the growth and development of microalgae of the Chlorella vulgaris BIN strain and associations of microorganisms that coexist in symbiosis with microalgae.
Key words: Chlorella vulgaris BIN, microorganisms, symbiosis, aquatic environment, heterotrophic organisms, hydrobitis
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