Wound healing is a normal human response which suddenly occurs after the injury. An inappropriate response of the healing process can result in the problem of chronic wounds. Nowadays, use of traditional herbal medicine for wound treatment is occurring popularly. It is getting to be important, showing a huge impact on the global health care system. Ya-RakSa-Phlae (YRSP) is a traditional Thai herbal recipe that has been traditionally used for wound treatment for a long time. However, there are no scientific researches confirming the efficiency of YRSP. This study was aimed to preliminarily investigate the related-wound biological and wound healing properties of the ethanolic extract of Ya-RakSa-Phlae recipe (E-YRSP) to prove its traditional property. E-YRSP possessed anti-bacterial activity with MIC 16-1,024 μg/mL against both Gram-positive and Gram-negative bacteria. Moreover, it was found to inhibit inflammation with IC50 up to 1,000 μg/mL and oxidation with IC50 ranged from 6-20 μg/mL. It was noted that activities of E-YRSP on anti-infection, anti-inflammation, and anti-oxidation markedly resulted in a competent wound healing efficacy. Phenolic compounds were abundantly found in E-YRSP as a large group of phytochemical constituents including catechin, rutin, demethoxycurcumin, luteolin, curcumin, and mangostin which might be a bioactive agent for wound healing promotion. This study has confirmed that YRSP obviously possessed strong wound healing activity as well as other biological properties associated with wound healing including, anti-oxidation, anti-inflammation, and anti-bacteria. Therefore, it is worthwhile to further study on its mechanisms on wound healing process and also to develop as an alternative treatment for wound treatment.
Key words: Anti-bacteria, Anti-inflammation, Anti-oxidation, Wound healing, Ya-RakSa-Phlae