Mastitis is a major production disease of dairy animals of global importance. A cross sectional study was conducted in and around Ambo town from November 2014 to April 2015 on lactating dairy cows to determine the prevalence of mastitis and associated risk factors. The study was done on 384 lactating cows, of which 120 were local and 264 cross breeds from dairy farms and small stockholders by using questionnaire, clinical examination, and California mastitis test (CMT). Out of 5 dairy farms and 68 small stockholders surveyed, there was no use of machine milking rather they used manual hand milking. Of the total 384 lactating dairy cows examined, 161(42%) cows were infected mastitis with 36 (9.4%) clinical mastitis and 125 (32.6%) subclinical mastitis. Of the potential associated intrinsic cows risk factors considered in this study, breed, parity, age, body condition and milk yield showed significant effects on prevalence of mastitis (p
Key words: California mastitis test, Dairy farms, Prevalence, Risk factors, Subclinical mastitis
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