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Original Article



Metabolic Effects of Cedar Tar on Colon Cancer Cell Line (HCT-116): A Follow-up Study

Ebru Temiz,Kadir Egi,Ismail Koyuncu,Ozgür Yüksekdag,Yusuf Kurt,Murat Tiken,Sükrü Akmese,Mehmet Enes,Yazan Awad.




Abstract
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Objective: Colon cancer (CC) is considered to be the most common cancers seen worldwide. Surgery and chemotherapy are the leading methods used in treatment. In recent years, the therapeutic properties of natural products have been investigated and used in drug development. Cedrus libani plant that normally grows in Syria and Turkey, some studies have shown that the plant’s extract (cedar tar) has anti-microbial and anti-cancer properties on colon cancer. Recently, metabolic changes have been identified as the hallmark of cancer, and various metabolic drugs are being investigated in clinical trials. This study was carried out to investigate the effects of cedar tar on the amino acid metabolism of colon cancer cells.
Materials and Methods: Colon cancer cells (HCT-116) were homogenized in cold PBS medium after incubation with 30 µg/mL dose of cedar tar for 24 hours in appropriate medium. The intracellular free amino acid profile in the samples was analyzed by LC-MS/MS method. Statistical analysis of the obtained data was done with SPSS and MetaboAnalyst 5.0 program.
Results: According to the statistical analysis results, significant positive correlations were found between β-alanine, anserine amino acids and apoptotic effects of cedar tar between the untreated control and cedar tar-treated groups, while a negative correlation was found with O-Phosphorylethanolamine amino acid.
Conclusion: By increasing the levels of β-alanine and anserine amino acids in colon cancer cells, cedar tar reduces both the metastatic character of the cells and inhibits tumor formation; It caused cell proliferation and energy deprivation by reducing the level of O-Phosphorylethanolamine amino acid, which is defined as a pro-tumorigenic metabolite. Cedar tar both triggered apoptosis and changed amino acid metabolism in colon cancer cells. It has been determined that cedar tar has the potential to be used as a natural product in the treatment of colon cancer.

Key words: Keywords: Amino Acide profile; Cedrus libani; Colon Cancer; LC-MS/MS






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