ABSTRACT
Cancer has been considered one of these major public health problems. Althaea ludwigii L. (Family: Malvaceae) is an indigenous plant, widely distributed in Iraq. The objective of this study is to investigate the antiproliferative activity of Althaea ludwigii L. on Breast cancer cell line MCF-7 and rate embryonic fibroblasts (REF) normal cell line, and to identify the bioactive constituent. The Arial parts of Althaea ludwigii L. were grinded into fine powder then extracted with 85% methanol. The extract was further partitioned with Ethyl acetate. Ethyl acetate fraction was chosen to test against MCF-7 by (3-[4, 5-dimethylthiazol-2-yl]-2, 5 assay (MTT assay) the bioactive constituents were investigated by high performance thin layer chromatography HPTLC. The data showed that ethyl acetate fraction has significant antiproliferative activity against MCF-7 (IC50 =35. 5µg/ml) and REF (IC50 =1762.9 µg/ml), HPTLC showed the existence of Rutin in Ethyl acetate fraction. The antiproliferative activity may relate to the existence of Rutin which had been approved as potent anti-cancer.
Key words: Cytotoxicity, Cellular viability, Plant extract, Flavonoids, Breast cancer
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