Abstract

The present study design to assess the qualitative phytochemical constituents responsible for anti-inflammatory, thrombolytic and cytotoxic activity of methanol extract of Caryota mitis leaves. The freshly collected C. mitis leaves extracted with methanol (MECM) to evaluate the secondary plant metabolites along with anti-inflammatory activity by protein denaturation assay. Thrombolytic and cytotoxic activity investigated using human blood clot lysis and brine shrimp lethality bioassay, respectively. The MECM exhibited several secondary plant metabolites named alkaloid, glycoside, protein, flavonoid, reducing sugar, saponins, and phenolic compound. The MECM extract (62.5-1000 μg/mL) exhibited an extremely significant (P < 0.0001) 6.06-45.45% inhibition of protein denaturation against positive control diclofenac sodium (43.11-85.48%). In thrombolytic, the MECM exhibited 24.29 % significant (P < 0.0001) protection against blood clotting, whereas the positive control streptokinase (75.35%). In the cytotoxic study, a significant (P < 0.05) percentage of mortality exhibited while 70% mortality of Brine shrimp after 24 h at a concentration of 1000 μg/mL with an LC50 value of 550.57 μg/mL whereas the positive control vincristine sulfate (1.63 μg/mL). The current results suggesting MECM has promising anti-inflammatory, thrombolytic activities with weak cytotoxicity.

Key words: Caryota mitis; anti-inflammatory; thrombolytic; cytotoxicity