Cordyceps militaris is famous for its medicinal effects and variety of bioactivities including antimicrobial, anti-inflammatory, antioxidant, immunomodulatory, or antitumor properties. The research’s objective is to look into the antioxidant and cytotoxic effects of C. militaris extract (CME) against normal human colorectal HT-29 cancer cell line. The effects of CME and fresh Cordyceps militaris (CM) on the antioxidant activities were determined using total phenolic content (TPC), total flavonoid content (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) analysis. The cytotoxic effects of various concentrations of CME on HT-29 cells were evaluated by 3-[4,5-dimethylthiazol-2-yl]- 2,5-diphenyltetrazolium bromide test. From the results, CME displayed strong activity of DPPH (83.8%, inhibitory concentration = 0.60 mg/ml), TPC (160 ± 0.74 mg gallic acid equivalent/100 g), and TFC (6.6 ± 1.13 mg rutin equivalent/100 g) relative to fresh CM. CME was found to be significantly more cytotoxic toward HT-29 cells with p < 0.001 in a dose-dependent manner with a cell growth inhibitory concentration of 50% of t1.53 mg/ml in contrast to cisplatin (3.11 mg/ml). The high antioxidant activities and cytotoxic effects of CME are probably due to the extract’s high phenolic and flavonoid content. According to this report, CME’s growth inhibitory activity on human HT-29 cells is driven by an apoptotic mechanism involved in it.
Cordyceps militaris, Cytotoxicity, Human colorectal HT-29 cancer cell, Antioxidant activities.
Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 209-220
Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue.
Curtis MA, Vedam-Mai V
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 201-208
Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling.
Bertucci T, Kakarla S, Kim D, Dai G
Methods in molecular biology (Clifton, N.J.). 2022; 2375(): 1-12
Highly sensitive detection of multiple proteins from single cells by MoS-FET biosensors.
Wei J, Zhao Z, Lan K, Wang Z, Qin G, Chen R
Talanta. 2022; 236(): 122839
Identifying Neural Progenitor Cells in the Adult Human Brain.
Park TIH, Waldvogel HJ, Montgomery JM, Mee EW, Bergin PS, Faull RLM, Dragunow M, Curtis MA
Methods in molecular biology (Clifton, N.J.). 2022; 2389(): 125-154