The silver nanoparticles (AgNPs) exhibit properties like non-toxicity, biocompatibility, and broad antimicrobial activity. The present study focused on the use of a biochemical method to synthesize the AgNPs using biosurfactants produced by Pseudomonas aeruginosa strain LTR1 inoculated in 2% soybean oil. A reverse micelle approach was used for obtaining microemulsions of biosurfactant-based aqueous AgNO3 and sodium borohydride. The mixture of such microemulsions yielded a solution of AgNPs. UV-visible spectrophotometer was utilized for the determination of signature spectra of nanoparticles, here AgNPs showing absorption at 435 nm. X-ray crystallography and scanning electron microscopy determined the size of nanoparticles to be in the range of 34.53 to 36.13 nm. The biosurfactant-based AgNPs were assessed for antimicrobial activity against pathogens like Escherichia coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus, Bacillus subtilis and Candida albicans.
Key words: Pseudomonas aeruginosa,Biosurfactant, Silver nanoparticles, SEM-EDX
|
