Objectives: To assess the expression of Cyclin D in transition of normal oral mucosa to oral squamous cell carcinoma (OSCC) and to find the possible association of immunostaining in various clinicopathological and histopathological distribution of OSCC.
Methodology: In this cross sectional analytical study, 120 diagnosed paraffin embedded blocks were included comprising of 60 samples of normal oral mucosa (Group 1) and 60 cases of various grades of OSCC (Group 2). All tissue samples belonged to tobacco chewers. PatientsÂ’ record files were studied for age, gender, tobacco habits and various clinicopathological details. Immunohistochemistry was performed using Cyclin D monoclonal antibodies on all samples. Staining with Cyclin D was done to find its possible association with various clinicopathological and histopathological variables. Association of Cyclin D expression was also observed in transition of normal mucosa to OSCC.
Results: In Group 1, 75% were negative for Cyclin D. In Group 2, 100% were positive for Cyclin D. We found significant association for Cyclin D staining in transition of normal oral mucosa to OSCC in tobacco users. Nonsignificant association was found in Cyclin D positivity in various grades and stages of OSCC.
Conclusion: The overall Cyclin D expression was shown not to be increased with increasing grades and stages of OSCC in tobacco users. Positive association was seen in Cyclin D immunopositivity with transition of healthy mucosa to OSCC among tobacco users.
Key words: Cyclin D, Normal oral mucosa, Oral squamous cell carcinoma.
Correction done :
KEY WORDS: Cyclin D, Normal oral mucosa, Oral squamous cell carcinoma. Immunohistochemistry
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