Diabetic wound healing impairment remains a major problem in diabetic patients. Medical plants and new products are subjected to have a powerful pharmacological treatment modality on wound healing as sesame oil and Chlorine dioxide gel. Herein, we assessed the contribution of both topical sesame oil and chlorine dioxide gel application on the early healing process of full-thickness wounds in alloxan diabetic rat models. A full-thickness (8mm) artificial uniform skin wounds were created in alloxan-induced diabetic rats (100mg/kg b.w.). Rats were randomly divided into four groups: groups treated with sesame oil and chlorine dioxide gel, non-treated groups served as control non-diabetic, and control diabetic non-treated. Antioxidant activity was evaluated, post healing biopsies were histopathologically and immunohistochemically assessed in addition to measuring inflammatory mediators (TNF-α) by PCR. We found higher blood glucose levels, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea, serum creatinine, and nitric oxide (NO) levels in diabetic groups in comparison to control non-diabetic ones. Low total protein and superoxidase dismutase (SOD) levels in control diabetic non-treated rats than the control nondiabetic and treated groups. A higher level of malondialdehyde (MDA) was detected in the control diabetic group compared to the other treated and control non-diabetic group. Treated wounds showed better contraction percentage, granulation tissue formation, tissue regeneration and lower inflammatory mediator level (TNF-α) compared to control diabetic un-treated wounds. Sesame oil and chlorine dioxide gel applications are able to accelerate wound healing activity in diabetic rats.
Key words: Alloxan, MDA, Rats, Regenerative medicine, Skin, TNF-α.
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