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Upsurge production of cellulase from maize stover under soildstate conditions mediated by Streptomyces enissocaesilis DQ026641

Ashok Sudarshan, Siddanna Renuka, Sirasagar Reshma, Bhalerao Shilanjali, Dayanand Agsar.


Abstract

Maize stover was explored as a sole substrate of carbon for the extra cellular production of cellulase under solid-state conditions by an actinobacterium, Streptomyces enissocaesilis DQ026641. The upsurge production of cellulase was optimized manually regulating important process variables such as substrate (particle size, moisture content), organism (inoculum size), physicochemical attributes (pH, temperature) and mineral salts (NaCl, MgSO4, FeSO4) following one factor at a time approach. The maximum production (71.10 IU/ml) of cellulase was obtained at particle size 2 mm, moisture content 1.25%, temperature 45°C, inoculum size 1 × 108 and NaCl 1.5%. Enhanced production (79 IU/ml) of cellulase was achieved by following Response Surface Methodology with Centre Composite Design, considering pH 6.5, temperature 45°C and sodium chloride 1.5% as critical process variables. The quadratic impact of pH, temperature and sodium chloride significantly contributed for the production of cellulase under solid-state conditions from maize stover mediated by S. enissocaesilis DQ026641.

Key words: Maize stover, Actinobacterium, Cellulase, Solid-state conditions and Enhanced production






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