Home|Journals|Articles by Year|Audio Abstracts
 

Original Article



Development and validation of LC-MS/MS method for alpelisib quantification in human plasma: Application to pharmacokinetics in healthy rabbits

Tandrima Majumder, Shiva Kumar Gubbiyappa.




Abstract
Cited by 1 Articles

A new sensitive and specific liquid chromatography-tandem mass spectrometry methodology was needed for the estimation of alpelisib in plasma samples. Drug components were extracted by liquid-liquid extraction technique utilizing ethyl acetate. The liquid chromatographic system was processed with Zorbax C18 (50 × 4.6 mm, 5 μ) reverse phase analytical column with an isocratic mobile solvent system consisting of acetonitrile and 0.1% formic acid in the ratio of 90:10 (%V/V). Analytes were detected on a mass system equipped with a triple quadrupole system and electrospray ionization, functioning in multiple reaction monitoring, with the transitions of m/z 442.15 → 70.06, m/z 409.14 → 391.13 for alpelisib, dapagliflozin, respectively, in the positive ionization mode. The method has given more than 90.0% recovery values and accuracy values were present between −4.32% and 4.37% of relative error. All the relative standard deviation findings were less than 4.21%. Alpelisib pharmacokinetic parameters were determined from the curve obtained by taking time on the X-axis and concentration of plasma on the Y-axis. Alpelisib has to mean Tmax of 2.0 ± 0.21 and AUC0→α, AUC0t, and mean Cmax, for test formulation is 3,624.21 ± 315, 3,075.20 ± 316, and 269 ± 12.17, respectively.

Key words: Alpelisib, Cancer, LC–MS/MS, Validation, Linearity, Pharmacokinetics.






Full-text options


Share this Article


Online Article Submission
• ejmanager.com




ejPort - eJManager.com
Refer & Earn
JournalList
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under Creative Commons Attribution 4.0 International License (CC BY), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.