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Enzyme Immunoassay for Determination of Plasma Total Estrogen in Black Bengal Goat during Peripubertal Period

Avijit Haldar, Aditi Patra, Chanchal K. Biswas, Bukkaaraya S. Prakash.


The current study aims the optimization and validation of an enzyme immunoassay (EIA) for the quantification of total estrogen (TE) in blood plasma of Black Bengal goats during peripubertal period. A microtiter plate based competitive assay was developed using a capture EIA for anti-bovine TE and horseradish peroxidase coupled bovine TE. Bovine TE standards were prepared in charcoal-dextran stripped goat plasma. Specificity: A dose-response inhibition curve resulting from serially diluted goat plasma was parallel to the standard curve using bovine TE and thus it confirmed the similar specificity of bovine TE standard and endogenous TE in goat plasma for bovine TE antibody. Sensitivity: The minimum detection level of the assay was 0.10 pg TE/ 50 ml, corresponding to 2 pg/ ml plasma. Precision: The inter- and intra-assay coefficients of variations (for repeatability and reproducibility, respectively) for the pooled plasma samples in 10 assays were 8.5% and 7.3%; 9.7% and 7.3%, respectively. Validation: Pubertal symptoms in goats were coincided with the higher plasma TE level. Plasma TE levels in the goats exposed with buck were higher (P

Key words: Black Bengal, Enzyme Immunoassay, Goat, Peripubetal Period, Total Estrogen

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