This study is the first report on the antimicrobial and cytotoxic activities of fungal extracts isolated from marine sponge Dactylospongia sp, which is collected from Mandeh Island, West Sumatra, Indonesia. The isolation of fungal was conducted using dilution method with Saboround Dextrose Agar (SDA) + chloramphenicol (0.05%) as medium. Pure isolated fungal was cultivated on rice medium at temperature 25-27°C, then extracted by using ethyl acetate solvent. The ethyl acetate extract of each isolated fungal were tested for antimicrobial and cytotoxic activities. Nine fungal strains have been isolated from this sponge. Two ethyl acetate extracts of fungal strains (Dc 03, and Dc 04) were categorized as having strong inhibition against the growth of Staphylococcus aureus, Escherichia coli, Methicillin-Resistant Staphylococcus aureus (MRSA) and Multi-Drug Resistant Pseudomonas aeruginosa (MDRPA) in a concentration of 5% with zone inhibition in range of 12.31 ± 0.54 16.14 ± 0.75 mm. The cytotoxic activity screening of the ethyl acetate extracts of fungal strains was done by using the Brine Shrimp Lethality Test (BSLT). Four fungal strains had LC50 below 80 µg/ml (Dc 03, Dc04, DC 05 and DC 08) and were further tested with MTT assay on T47D cell lines.These selected fungi were identified molecularly as Cladosporium halotolerans MN859971, Penicillium citrinum MN859968, Aspergillus versicolor MN859970 and Aspergillus sydowii MN859969, respectively. The results suggest that these fungal strains are quite rich in the production of bioactive compounds that are very effective as antibacterial and cytotoxic agents.
Key words: marine sponge, Dactylospongia sp, marine derived-fungi, antibacterial activity, cytotoxic activity, Methicillin-Resistant Staphylococcus aureus (MRSA), Multi-Drug Resistant Pseudomonas aeruginosa