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Original Article

ECB. 2016; 5(6): 225-231


FUNGAL STRAIN OF ASPERGILLUS ORYZAE IMMOBILIZED ON SILICA GEL FOR Au(III) SORPTION

Dnyaneshwar K. Kulal, Amol V. Pansare, Amol A. Shedge, Vishwanath R. Patil.




Abstract

Biomasses have significant impact on the development of new solid phase extraction methods for metal analysis. Adsorbent, prepared by
incorporating dried biomass (Aspergillus oryzae) immobilized on activated silica, was presently developed for Au(III) enrichment. The
main factors affecting the adsorption-desorption process like pH, sample volume, eluent and eluent flow rate were optimized. Under the
optimum conditions, good recovery was obtained at about 99.4 ± 0.1 %, performed by Flame Atomic Absorption Spectrometry. At
optimized pH 2.0, Au(III) was quantitatively sorbed and recovered with 1:1 mixture of 0.2 M HCl mixed with 0.2 M thiourea. The
enrichment factor was found as 125, and the limit of detection is 0.88 µg L-1 whereas limit of quantification is 2.93 µg L-1. The proposed
method was successfully applied in the analysis of tap water, pharmaceutical formulated product and synthetically prepared dummy
sample. Equilibrium data also were fitted by linear regression methods through Langmuir, Freundlich, Temkin, Dubinin and
Radushkevich (D–R) and Harkin –Jura isotherm models.

Key words: Pharmaceutical formulated product, Aspergillus oryzae, immobilization, atomic absorption spectroscopy, solid phase extraction.






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