Breast cancer is a disease caused by abnormal cell proliferation in the breast. Gods crown fruit (Phaleria marcocarpa) and its seed has potential as an antiproliferation of cancer cells. It contains active compounds such as flavonoids, alkaloids, polyphenols, and tannins. The sample of gods crown fruit was obtained by extraction and fractionation using maceration method. Cytotoxicity of extracts and fractions were determined using Brine Shrimp Lethality Test (BSLT) method. Antiproliferation activity test of gods crown fruit against MCM-B2 using the hemasitometer method. The gods crown fruit sample consist of crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction. LC50 values in crude ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction were 13.72 ppm, 147.55 ppm, 405.81 ppm, and 149.55 ppm respectively. The concentration of the test sample was directly used for the antiproliferation activity test on MCM-B2 cells. Gods crown fruit can act as antiproliferation of MCM-B2. The smallest concentration of those sample was inhibit MCM-B2 cell proliferation is 3.5 ppm crude ethanol extract that lower than 100 ppm doxorubicin. The maximum percentage of antiproliferation activity of crude ethanol extract (56 ppm) was able to inhibit MCM-B2 cell proliferation by 58.28% while doxorubicin (100 ppm) by 31.2%. This is due to crude ethanol extract has a lot of complex polar phytochemical content. The crude ethanol extract compounds inhibit of MCM-B2 cells proliferation synergistically.
Key words: antiproliferation, breast cancer, cytotoxicity, MCM-B2 cells, Phaleria marcocarpa