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Original Article

Prednisolone encapsulated PLGA nanoparticles: Characterization, cytotoxicity and anti-inflammatory activity on C6 glial cells

Sriprasad Acharya, Bharath Raja Guru.

Prednisolone, a popular glucocorticoid known for its ability to inhibit cytokine release is also employed extensively in cancer therapy. Glucocorticoids have been used to treat brain tumors to reduce tumor associated edema. They are known to exhibit different effects on different cell lines and in some cases are known to be neurotoxic. In this study we have investigated and compared the cytotoxic and anti-inflammatory effects of prednisolone and prednisolone encapsulated Poly Lactic-co-Glycolic acid (PLGA) nanoparticles (NPs) on C6 cells that are cancerous but are known for their similarity to astrocyte cells. Design expert software was used to analyze the effect of different variables for nanoparticle formulation. By varying different parameters nanoparticles were synthesized and characterized using particle size analyzer, zeta potential. Surface morphology of the NPs were analyzed using scanning electron microscopy (SEM). Lipopolysaccharide (LPS) activated C6 glial cells witnessed significantly lower cell proliferation in the presence of the drug during the 48 h incubation and the prednisolone encapsulated NPs were able to attenuate pro-inflammatory cytokines like TNF-α and Nitric oxide (NO) substantially even after the 72 h of incubation when compared it with free drug. The results suggested that prednisolone was more effective as an anti-inflammatory drug in the PLGA nanoformulation.

Key words: Prednisolone nanoparticles, anti-inflammatory, cytotoxicity, Factorial design

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