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Role of N-acetyl cysteine on bone marrow-derived hematopoietic stem/progenitor cell cryopreservation: Outcome on the oxidative stress-mediated cryodamage and repopulation Capacity into hematopoietic lineages

Zariyantey Abdul Hamid, Preshetha Sellappah, Hemabarathy Bharatham, Ramya Dewi Mathialagan, Vayithiswary Dewi Kannan.




Abstract

Cryopreservation of hematopoietic stem / progenitor cells (HSPCs) is associated with oxidative stress-mediated cryodamage; hence compromising the therapeutic potency. The roles of N-acetyl cysteine (NAC) on the oxidative stress-mediated cryodamage and repopulation capacity of HSPCs into myeloid, erythroid and pre-B lymphoid progenitors were investigated. Mice bone marrow-derived HSPCs were cultured for 24 hours, followed by cryopreservation at 1 x 106 / ml cells in cryomedium containing 10% DMSO with NAC (0.25, 0.5 or 2.0 µM) or without for 48 hours, 2 weeks and 4 weeks at -80°C. Cryopreservation significantly reduced cell viability at post-thawed (p

Key words: Hematopoietic stem/progenitor cell, cryopreservation, antioxidant, N-acetylcysteine, oxidative stress, repopulation capacity.






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