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Original Article

AJVS. 2021; 69(1): 43-51


Biofilm Formation and Overcoming Strategies of E.Coli Isolated from Human and Chickens

Ahmed M. Elshwehy, Samy A. Khalil, Helmy A. Turky, Rasha G.Tawfik.

Abstract
E. coli is one of the commonest causes of bacteremia in humans around the world, accounting for 15% - 40% of all significant bacteremia isolates. The present study aimed to assess the role of aspirin as an overcoming strategy for biofilm formation in E. coli isolated from humans urinary tract infection samples and chickens. A total number of 435 samples collected (200 from human urine samples and 235 chickens) from Alexandria governorate. The obtained results revealed that E.coli was isolated from 158 out of 200 (79%) human samples and 110 out of 235 (46.8 %) samples from chickens. A total number of 81 isolates were evaluated for biofilm formation (50 isolates from human and 31 from chickens). Forty-five isolates out of 50 isolates (90%) from human urine and 19 out of 31 isolates (61.3%) from chickens revealed the presence of biofilm in 19 samples with percentage of 61.29%. Twenty-six isolates were tested by real time PCR (RT-PCR) for evaluation of ndvb gene expression. Twenty isolates positive for biofilm formation were treated with Aspirin from human urine samples and 6 isolates from chicken samples. Six isolates negative for biofilm formation were used as a negative control. It could be concluded that there is a relation between biofilm formation and antibiotic resistance. Treatment of the isolated E.coli with aspirin reduced biofilm formation and increase sensitivity of E.coli to antibiotics. On conclusion aspirin may be used as an adjuvant to antibiotics in the management of drug resistant E. coli infections. However, more studies conducting the same perspective should be conducted in the near future

Key words: E. coli , Biofilm, , ndvb gene, Human, Chickens



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