| Short Communication | 
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Dual natural infection with bovine viral diarrhea virus (BVDV)-1 and BVDV-2 in a stillborn calf: tissue distribution and molecular characterizationMaximiliano Joaquín Spetter, Enrique Leopoldo Louge Uriarte, Erika Analía González Altamiranda, María Rosa Leunda, Susana Beatriz Pereyra, Andrea Elizabeth Verna, Anselmo Carlos Odeón. Abstract | | | | Dual infections with both bovine viral diarrhea virus (BVDV)-1 and -2 seem to be unusual. The aim of this study was to describe an infection with both BVDV genotypes in a stillborn calf. Virus isolation and phylogenetic analyses of the 5´UTR and NS5B regions confirmed the presence of BVDV-1b and -2b in spleen and lung, whereas BVDV-2b was also detected in brain, heart, liver, kidney and, fluid of cavities. These results confirm that dual infections with both BVDV-1 and BVDV-2 species can occur naturally and their tissue distribution can be different.
Key words: bovine viral diarrhea virus, co-infection, phylogenetic analysis
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