The α,β-unsaturated δ-lactone as antitumor activity has been reviewed. Hyptolide was isolated from the leaves of Hyptis pectinata Poit (Lamiaceae) and was studied in order to discovery and developed anticancer drug. Hyptolide was obtained as crystal of 87-880C melting point. Spectroscopic identification results showed wavenumber at1735 cm-1 indicated α,β-unsaturated δ-lactone presence. GC-MS analysis provide single peak with 11.701 retention time as well as m/z value at 239 unique for hyptolide. The objective of this research was to evaluate the cytotoxic mechanism under hyptolide on human breast cancer MCF-7 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-dphenyl tetrazolium bromide (MTT) assay showed that hyptolide exhibited cytotoxic effects on MCF-7 and T47D breast cancer cells, with IC50 value of 76.76 µg/mL and 181.55 µg/mL, respectively. Intersetingly, treatment of hyptolide for 24, 48 and 72 h decreased cell viability on MCF-7 with dose and time dependent manner compared to untreated cells. Results of acrydine orange-ethidium bromide (AO/EB) staining assay revealed that hyptolide induced apoptosis in dose-dependent manner. The results concluded that hyptolide possesses antiproliferative effects through apoptosis induction.
Key words: Hyptis pectinata Poit, hyptolide, cytotoxic, apoptosis, antiproliferative, MCF-7 cells