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Acta Inform Med. 2008; 16(2): 86-87


Detection of Human Coxiella Burnetii Infection Using Indirect Immunofluorescence Assay (IFA)

Sadeta Hamzic, Edina Beslagic, Sukrija Zvizdic.




Abstract

The examination involved the individuals, whose sera were tested in the Laboratory for specific diagnostic of human Q fever, in the Department for Microbiology of the Medical Faculty University of Sarajevo, during the period 2000 – 2004, using the IFA test for detection of human IgM and IgG antibodies for C. burnetti. Human Coxiella burnetti infection was confirmed in 191 (69.7%) men and 83 (30.3%) women, with the most frequent occurrence in the group aged between 31 and 40 (26.7%). Analyzing the values of obtained results of titers of specific IgM and IgG antibodies phase I and phase II C. burnetii antigens in sera of individuals involved, we confirmed the presence of acute disease in 80.3% of cases, as well as chronic, i.e. convalescent disease in 19.7% of cases.

Key words: Coxiella burnetii, specific human IgM and IgG antibodies, indirect immunofluroescence assay (IFA).






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