Alcohol tolerant microbes are the prime requirement for industrial scale production of biofuels and beverages. Tolerance is a complex phenomenon that is achieved by mutational changes at several points in the genome. Since a network of genes and pathways are involved in adapting to ethanol tolerance it is therefore more preferable to obtain ethanol tolerance phenotype by adaptive evolution. Adaptive evolution ensures genotypic changes which results in evolution of a phenotypically tolerant strain. In present work, Lysinibacillus sp. isolated from estuarine area was subjected to adaptive evolution under ethanol stress that resulted in an increase in ethanol tolerance from 1.6% to 6.4%.
Key words: Ethanol, tolerance, Lysinibacillus, antibiotics, estuary
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