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Original Article

ECB. 2015; 4(3): 130-134


GENETICALLY ENGINEERED ESCHERICHIA COLI FBR5 TO USE CELLULOSIC SUGARS: PRODUCTION OF ETHANOL FROM CORN FIBER HYDROLYZATE EMPLOYING COMMERCIAL NUTRIENT MEDIUM

N. Qureshi, B. S. Dien, B. C. Saha, L. Iten, S. Liu, S. R. Hughes.




Abstract

Transportation biofuel ethanol was produced from xylose and corn fiber hydrolyzate (CFH) in a batch reactor employing Escherichia coli
FBR5. This strain was previously developed in our laboratory to use cellulosic sugars. The culture can produce up to 49.32 g L-1
ethanol
from approximately 125 g L-1 xylose. Use of commercial nutrient sources such as corn steep liquor (CSL) and soy peptone (SP) was also
studied and SP was found to be superior than CSL. SP at a concentration of 15 g L-1
resulted in the production of 42.2 g L-1
ethanol with
ethanol yield and productivity of 0.49 and 0.74 g L-1 h
-1
, respectively. Corn fiber (CF) was pretreated with dilute H2SO4 and hydrolyzed
using commercial cellulases. Employing CFH as a sole substrate, the culture produced 35.33 g L-1
ethanol with a productivity and yield of
1.01 g L-1 h
-1
and 0.54, respectively, leaving behind no residual sugars in the medium. This productivity is 40 % higher than when using
xylose as the carbon source in the control experiment.

Key words: corn fiber, corn fiber hydrolyzate, ethanol, productivity, yield, Escherichia coli FBR5






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