Aim: To investigate anticancer effect of Sesamum Indicum extract against breast cancer cell line ( MCF -7).
Introduction: MCF-7 is a breast cancer cell line. Breast cancer is the malignant tumor that starts in the cells of the breast .Among Indian women, breast cancer is the commonest cancer in Indian women. Sesamum Indicum ( Pedaliaceae) is a plant growing and cultivated in India etc. This plant is used for some medicinal uses such as digestive ,sedative, tonic, diuretic as well as for functional GIT disorders.
MCF-7 is a breast cancer cell line. Breast cancer is the malignant tumor that starts in the cells of the breast .Among Indian women, breast cancer is the commonest cancer in Indian women. Sesamum Indicum ( Pedaliaceae) is a plant growing and cultivated in India etc. This plant is used for some medicinal uses such as digestive ,sedative, tonic, diuretic as well as for functional GIT disorders.
Materials and Method: The plant materials S. indicum powder was purchased from Life care phytolab Private limited.
Cytotoxicity assay on MCF7 cell line Chemicals and reagents: MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) invitrogen,USA. Acridine orange were obtained from Sigma, USA. All other fine chemicals were obtained from SigmaAldrich, St. Louis.
Cell culture: MCF-7 cells obtained from NCCS (National Centre For Cell Science, Pune) were cultured in Rose well Park Memorial Institute medium (RPMI), supplemented with 10% fetal bovine serum, penicillin/streptomycin (250 U/mL), gentamycin (100μg/mL) andamphotericin B (1mg/mL) were obtained from Sigma Chemicals, MO, USA. All cell cultures were maintained at 370C in a humidified atmosphere of 5% CO2. Cells were allowed to grow to confluence over 24 h before use.
Cell growth inhibition studies by MTT assay: Cells (1 × 105/well) were plated in 24-well plates and incubated in 370C with 5% CO2 condition. After the cell reaches the confluence, the various concentrations of the samples were added and incubated for 24hrs. After incubation, the sample was removed from the well and washed with phosphate-buffered saline (pH 7.4) or MEM without serum. 100µl/well (5mg/ml) of 0.5% 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl--tetrazolium bromide (MTT) was added and incubated for 4 hours. After incubation, 1ml of DMSO was added in all the wells .The absorbance at 570nm was measured with UV- Spectrophotometer using DMSO as the blank. Measurements were performed and the concentration required for a 50% inhibition (IC50) was determined graphically. The % cell viability was calculated using the following formula:
% cell viability = A570 of treated cells / A570 of control cells × 100
Graphs are plotted using the % of Cell Viability at Y-axis and concentration of the sample in X-axis. Cell control and sample control is included in each assay to compare the full cell viability assessments
Conclusion: The present study is to demonstrate the toxicity of the extract Sesamum indicum on MCF -cell lines. Cytotoxicity, induction of cell cycle arrest and apoptosis probably constitute the antitumour mechanisms of extract. From the research we identified that Sesamum Indicum has anticancer effect and is used to treat breast cancer.
Key words: Cell culture, cancer cell lines, Sesamum Indicum, cytotoxity, cell viability.