Background: Although the antihyperglycemic activity of stem extracts of Anisopus mannii (AM; an antidiabetic ethnomedicinal plant utilized in Northwestern Nigeria) has been reported, the effects of coadministration of the extracts with glibenclamide, a commonly used antihyperglycemic agent, are not known.
Aims and Objectives: The objectives were to determine the effect of simultaneous administration of AM methanolic stem extract with glibenclamide using streptozotocin/nicotinamide-induced diabetic rats.
Materials and Methods: After permission from the departmental ethics committee, male Wistar rats were assigned to five groups: Vehicle-treated normal control; vehicle-treated diabetic control; diabetic rats treated with either glibenclamide (0.6 mg/kg) or methanolic stem extract of AM (200 mg/kg), and singly or in combination. Fasting blood glucose (FBG) and oral glucose tolerance test (OGTT) were performed at days 14 and 28 of treatment, while lipid profile and the contents of hepatic and skeletal muscle glycogen were determined at the end of the study (day 29).
Results: When combined, the AM extract significantly reduced (P < 0.05) the effect of glibenclamide in lowering the total area under the OGTT curve as well as the FBG level and in increasing the contents of hepatic and skeletal muscle glycogen as well as the blood level of high-density lipoprotein cholesterol. The two treatments also significantly reduced each others effect in lowering triglycerides, low-density lipoprotein, and total cholesterol.
Conclusion: The methanolic stem extract of AM (200 mg/kg) combined with glibenclamide (0.6 mg/kg) reduced each others beneficial effects on glucose homeostasis and lipid profile in streptozotocin/nicotinamide-induced diabetic rats.
Key words: Anisopus mannii; Glibenclamide; Streptozotocin; Rats; Combination
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