In 2017. World Health Organization published its first-ever list of antimicrobial-resistant bacteria "priority pathogens," a catalogue of 12 families of bacteria posing the greatest threat to human health. This list focuses on the risk of Gram-negative bacteria for multiple drug-resistant. Pseudomonas aeruginosa was at the top of the list and critical. A current study aiming to demonstrate the prevalence of -β-lactamase among multidrug-resistant P. aeruginosa strains isolated from burn wound patients phenotypically. The isolates were identified and examined for susceptibility against ten antipseudomonal agents, and Screening β-lactamase (ESβLs, MβLs, and Amp-C) production phenotypic by combined diffusion disc test, Imipenem-EDTA. Results In the current study identified 98 P. aeruginosa isolates from 200 clinical specimens obtained from burn wound patients. Our result showed 65 (66.3%) of the 98 P. aeruginosa isolates were MDR strains. Out of 65 isolates, 37 (56.9%), 21(32.3%), and 40 (61.5%) were ESβLs, MβLs, Amp-C producing P. aeruginosa respectively according to Phenotypic detection method. We found co-expression of various β-lactamases In the present study, 16 isolates showed co-existence of AmpC+ESBL, 16 isolates were having ESBL+MBL+AmpC, and five isolates were having co-existence of ESBL+MBL. The occurrence of ESβLs, MβLs, Amp-C producing P. aeruginosa was demonstrated, calling for Phenotypical determination of antibiotic resistance mechanisms should be performed regularly to guide antibiotic selection during therapy. Significant conclusions drawn from this work include a rise in the rate of β-lactamase (ESβLs, MβLs, and Amp-C) in MDR P. aeruginosa. Later research should, therefore, focused on the study of Molecular Characterization.
Key words: Pseudomonas aeruginosa, burn wound, β-lactamase (ESβLs, MβLs, and Amp-C), Yemen
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