Objective: To detect biofilm development and to see the culture and sensitivity on DJ stent in obstructive uropathy.
Methodology: This descriptive cross sectional study was conducted at Urology, Pathology and Microbiology Department of Chandka Medical College Hospital SMBBMU Larkana and SALU Khairpur from January 1, to July 31, 2018. DJ stents were removed from all patients either General or in Local Anesthesia with the help of cystoscope and foreign body forceps. Lower part of stent was cut for biofilm. Lower part of DJ stent was cleared with normal saline to eradicate surface contaminants then shifted to tryptic soy broth to see culture and sensitivity.
Results: Out of 55 patients, 35 were male and 20 females. Mean age of males was 27±13.1 and female 22±13.8 years. Among them, 15(27.27%) were found to form biofilm, which specially occurred in those patient who had DJ for longer than six weeks. Biofilm formation was seen in 10 out of 35 (28.57%) males 5 out of 20 (25%) females. Stent culture was positive in 22 out of 55 (40%) patients. It was positive in 14 out of 35 (40%) in males 8 out of 20 (40%) females. The most common organisms seen were Escherichia coli in 15 (68.18%), Pseudomonas in 2(13.3%), Klebsiella in 2(13.3%), Enterococci in 1(6.6%) and Staphylococcus coagulase negative in 2(13.3%) patients.
Conclusion: Biofilm formation and positive cultures are common in any period of time, so removal of DJ stent should be well-planned with appropriate antibiotic prophylaxis to avoid complications like systematic inflammatory response syndrome and urosepsis.
DJ stent, Biofilm development, Culture and sensitivity
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