Home|Journals|Articles by Year Follow on Twitter

Directory for Medical Articles
 

Open Access

Research Article



Prevalence and antimicrobial susceptibility profiles of Staphylococcus aureus from raw bovine milk in dairy and pastoral farms in Morogoro region, Tanzania

Nancy Evans Kalee, Noel Gahamanyi, Abubakar Hoza.

Abstract
Increased resistance of Staphylococcus aureus isolates to existing antimicrobials constitutes a major concern in human and veterinary medicine. This study aimed at determining the prevalence, antimicrobial susceptibility profiles, and molecular characteristics of S. aureus from raw bovine milk in dairy and pastoral farms in Morogoro urban and Mvomero districts, Tanzania. In a cross-sectional study, 397 raw bovine milk samples were collected and carried to the laboratory. Conventional Gram staining, colony morphology on blood agar, and mannitol salt agar, along with biochemical tests, were used for S. aureus identification. Antimicrobial susceptibility testing (AST) was performed using the disk diffusion method, while multiplex polymerase chain reaction (PCR) was used to detect virulence and antimicrobial resistance genes. Data were analyzed using Epi Info (Version 7). Out of the 397 samples, S. aureus was confirmed in 124 (31.2%). Contamination of raw bovine milk by S. aureus in the study area was associated with poor hygienic measures. The AST revealed that all S. aureus isolates were susceptible to chloramphenicol and cefoxitin, while the highest resistance 116/124 (93.5%) was noticed for penicillin. Resistance to other antimicrobials varied between 1.6-28.2%. Of the 124 S. aureus isolates, 80 (64.5%) possessed spa gene, with 76/80(95.0%) being pathogenic. One of the S. aureus isolates, 1/124 (0.8%), harbored a mecA resistance gene. The presence of antimicrobial-resistant S. aureus isolates in raw bovine milk at the farm level is alarming and requires herd health improvement interventions to protect society.

Key words: Milk, Morogoro, Tanzania, virulence, mecA, Staphylococcus aureus



Similar Articles

Simultaneous preconcentration and determination of sulfonamide antibiotics in milk and yoghurt by dynamic pH junction focusing coupled with capillary electrophoresis.
Semail NF, Abdul Keyon AS, Saad B, Kamaruzaman S, Mohamad Zain NN, Lim V, Miskam M, Wan Abdullah WN, Yahaya N, Chen DDY
Talanta. 2022; 236(): 122833

Evaluation of alginate-biopolymers (protein, hydrocolloid, starch) composite microgels prepared by the spray aerosol technique as a carrier for green tea polyphenols.
Ramdhan T, Hung Ching S, Prakash S, Bhandari B
Food chemistry. 2022; 371(): 131382

Iodine deficiency disturbs the metabolic profile and elemental composition of human breast milk.
Arias-Borrego A, Velasco I, Gómez-Ariza JL, García-Barrera T
Food chemistry. 2022; 371(): 131329

Diversification of plasmids in a genus of pathogenic and nitrogen-fixing bacteria.
Weisberg AJ, Miller M, Ream W, Grünwald NJ, Chang JH
Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 2022; 377(1842): 20200466

Fluorescence detection of Staphylococcus aureus using vancomycin functionalized magnetic beads combined with rolling circle amplification in fruit juice.
Wang Y, Wang Z, Zhan Z, Liu J, Deng T, Xu H
Analytica chimica acta. 2022; 1189(): 339213


Full-text options


Latest Statistics about COVID-19
• pubstat.org


Add your Article(s) to Indexes
• citeindex.org






Covid-19 Trends and Statistics
ScopeMed.com
CiteIndex.org
CancerLine
FoodsLine
PhytoMedline
Follow ScopeMed on Twitter
Author Tools
eJPort Journal Hosting
About BiblioMed
License Information
Terms & Conditions
Privacy Policy
Contact Us

The articles in Bibliomed are open access articles licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
ScopeMed is a Database Service for Scientific Publications. Copyright ScopeMed Information Services.



ScopeMed Web Sites